Journal: Cell reports

Article Title: Proteasome activity maintains cell-type-specific gene expression

doi: 10.1016/j.celrep.2026.116973

Figure Lengend Snippet: (A) Replot of , proteins with statistically different changes in protein expression are colored by whether they are tissue-specific (red), not tissue-specific (blue), or not present in the RNA-seq database used for tissue-specificity determination (NA, not available, black). (B) Heatmap of the normalized transcript per million (nTPM) values across 50 different tissue types for the most significantly affected proteins (|log 2 | ≥ 2) in (A). For each protein, nTPM values were normalized to the highest nTPM value across all tissues. Color scale indicates the normalized nTPM values, with white representing lower and red representing higher values as indicated. (C–E) Immunoblots of lysate from HCT116 WT and hRpn10 VWA detecting PALM3, SUSD2, CDK6, SEC31A, RAB25, S100A14, hRpn10, and β-actin. (F) Model of proteasome activity contributing to cell identity that is represented by colored spots inside squares. A cartoon of a cell with the cytosol (yellow) and nucleus (light yellow) is displayed and an aberrant proteome (represented by little color variance) for cells with hRpn10 VWA proteasomes contrasted with a healthy proteome for cells with WT proteasomes. See also and .

Article Snippet: TaqMan probe for CDK6 , Applied Biosystem , Hs01026371_m1.

Techniques: Expressing, RNA Sequencing, Western Blot, Activity Assay

Journal: bioRxiv

Article Title: CDK6-mediated endothelial cell cycle acceleration drives arteriovenous malformations in hereditary hemorrhagic telangiectasia

doi: 10.1101/2023.09.15.554413

Figure Lengend Snippet: ( A ) Heatmap analysis of a high-throughput cell cycle protein ELISA of ECs isolated from livers of P6 BMP9/10ib mice vs. PBS control littermates. Data represent n=2 independent analyses (n represents one litter of pups combined for each condition; PBS, n=6 mice; BMP9/10ib, n=7 mice). ( B ) qPCR analysis of ECs isolated from livers of P6 PBS and BMP9/10ib mice (n=5/condition). Data represent mean ± SEM, Mann-Whitney test ( Cdk2 ), and unpaired t-test ( Cdk4 and Cdk6 ). ( C ) Flow cytometry quantification of p-RB1 fluorescence intensity in ECs isolated from livers of P6 PBS and BMP9/10ib mice (n=3/condition). Data represent mean ± SEM, unpaired t-test. *P < 0.05. ( D and E ) Representative IF staining of p-RB1 (green) and IB4 (red) in the peri-optic nerve and mid-plexus regions ( D ) and corresponding quantification of the peri-optic nerve region ( E ) of retinas from vehicle-treated PBS (n=6), palbociclib-treated PBS (n=5), vehicle-treated BMP9/10ib (n=6), and palbociclib-treated BMP9/10ib (n=6) mice. Data in ( E ) represent individual retinas and mean ± SEM, one-way ANOVA with Tukey’s multiple comparisons test. ns, not significant; *P < 0.05, **P < 0.01. Scale bars in ( D ), 50 μm. ( F ) Representative H&E and IHC staining of p-RB1 in 4 μm skin sections of HHT2 patients. Te, telangiectasia; N, normal vessel; Ba, basilar cells.

Article Snippet: Eng f/f , and Cdk6 f/f mice were crossed with Cdh5 -Cre ERT2 mice [Taconic, #13073; ] to generate EC-specific, tamoxifen-inducible KO mice ( Eng iECKO and Cdk6 iECKO ).

Techniques: High Throughput Screening Assay, Enzyme-linked Immunosorbent Assay, Isolation, Control, MANN-WHITNEY, Flow Cytometry, Fluorescence, Staining, Immunohistochemistry

Journal: bioRxiv

Article Title: CDK6-mediated endothelial cell cycle acceleration drives arteriovenous malformations in hereditary hemorrhagic telangiectasia

doi: 10.1101/2023.09.15.554413

Figure Lengend Snippet: ( A ) Representative staining using IB4 (green) and of SMA (red) in whole petals (first two rows), the vein front (third row), and peri-optic nerve (forth row) and mid-plexus (last two rows) regions of retinas from Cdk6 f/f controls and Cdk6 iECKO mice challenged or not (PBS) with BMP9/10ib. Arrows denote AVMs; a, artery; v, vein. Scale bars, 1 mm (whole petal images), 100 μm (vein front), and 50 μm (mid-plexus and peri-optic nerve areas). ( B-D ) Scatter plots showing retinal AVM number ( B ), vein diameter ( C ), and mid-plexus vascular density ( D ) in Cdk6 f/f ;BMP9/10ib controls (n=11-14) and Cdk6 iECKO ;BMP9/10ib (n=8-12) mice. Data represent individual retinas and mean ± SEM, unpaired t-test. **P 0.01, ***P 0.001. ( D ) Schematic illustration of the proposed mechanism of control of the cell cycle in ECs by ALK1 signaling and its relevance for HHT pathogenesis.

Article Snippet: Eng f/f , and Cdk6 f/f mice were crossed with Cdh5 -Cre ERT2 mice [Taconic, #13073; ] to generate EC-specific, tamoxifen-inducible KO mice ( Eng iECKO and Cdk6 iECKO ).

Techniques: Staining, Control

Journal: Cell stem cell

Article Title: Restraining Lysosomal Activity Preserves Hematopoietic Stem Cell Quiescence and Potency

doi: 10.1016/j.stem.2020.01.013

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-Rabbit polyclonal CDK6 , Novus biological , NBP187262.

Techniques: Recombinant, Staining, ATP Bioluminescent Assay, SYBR Green Assay, Reverse Transcription, RNA Sequencing, Software